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A search engine for N-linked intact glycopeptide identification in HCD tandem mass spectrometry experiment.

Version 2.1 (2019)

  1. Update the isotopic peaks validation

Version 2.0 (2018)

  1. Rewrite the software in pure Python language

  2. Use fragment ion index to speed up searching

  3. Add support to use glycopeptide sequences as well as spectral library

Version 1.0 (2015)
Build spectral library for N-linked Glycopeptide identification (Matlab)

Leaders: Yingwei Hu (, Shadi Toghi Eshghi

Other contributors: Minghui Ao, Michael Schnaubelt 
Please send email to, if you need older releases of GPQuest.

Related Publication:

  1. Reanalysis of global proteomic and phosphoproteomic data identified a large number of glycopeptides,
    Yingwei Hu, Punit Shah, David J Clark, Minghui Ao, Hui Zhang, Analytical chemistry 90 (13), 8065-8071, 2018

  2. Classification of Tandem Mass Spectra for Identification of N-and O-linked Glycopeptides,
    Shadi Toghi Eshghi, Weiming Yang, Yingwei Hu, Punit Shah, Shisheng Sun, Xingde Li, Hui Zhang, Scientific Reports 6, 37189,2016

  3. GPQuest: a spectral library matching algorithm for site-specific assignment of tandem mass spectra to intact N-glycopeptides,
    Shadi Toghi Eshghi, Punit Shah, Weiming Yang, Xingde Li, Hui Zhang, Analytical chemistry 87 (10), 5181-5188, 2015


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